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Alternaria leaf spot is one of the devastating diseases of cabbage caused by Alternaria brassicola. The experiment was conducted to understand the most effective culture media for the growth and multiplication of disease pathogens. Eight different culture media and four replications of each were employed under aseptic conditions for 12 days. Potato Dextrose Agar showed the highest radial growth (88.90mm) with a brownish to greenish colony color followed by Malt Extract Agar (87.45mm) with a whitish irregular margin of the dark brown colony. Sabouraud Dextrose Agar and Corn Meal Agar showed regular margins in their colony whereas Oat Meal Agar and Potato sucrose Agar showed irregular ones. Carrot Agar showed the lowest radial growth (53.12mm) with dark brown colony color at the center and dull reddish brown towards the margin which was followed by Nutrient Agar (59.62mm). This study will be a guide for further research on the physiology, taxonomy, and management of disease pathogens.