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Proper nitrogen management in Gladiolus is crucial for optimal development and productivity. Balancing nutrient supply is essential for vigorous vegetative growth, quality spikes, and abundant florets, maximizing yield without causing environmental harm. This experiment was done to assess the effect of different doses of nitrogen on gladiolus (Gladiolus grandiflorus L.) cv. American Beauty production in Chitwan. The randomized complete block design (RCBD) method was carried out at the horticultural farm of Nepal Polytechnic Institute (NPI), Bharatpur, Chitwan, from November 2021 to April 2022. Medium-sized corms (4–5 cm) of gladiolus were selected, planted, and subjected to five different levels of nitrogen treatments, i.e., 30 gm/m2 (T2), 40 gm/m2 (T3), 50 gm/m2 (T4), 60 gm/m2 (T5), and control (T1), and replicated four times. Observations were recorded for both vegetative and floral parameters. Results show that different doses of nitrogen significantly affected plant height, number of leaves, leaf length, spike emergence, spike length, number of florets per spike, number of corms and cormels, corm’s size, and weight. The highest plant height (102.45 cm), highest leaf length (38.30 cm), highest number of leaves per plant (7.75), highest spike length (80.40 cm), maximum number of florets per spike (15.40), maximum number of corm per plant (1.62), weight of corm (59.09 gm), and size of corm (5.73 cm) were observed at 50 gm N/m2 (T4). The late spike emergence (92.20 days) was recorded at 50 gm N/m2 (T4). The result shows that using 50 gm N/m2 can improve the growth, flowering, and corm production of gladiolus.