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This study aims to compare between the effects of soaking and/or spraying by chitosan (Chs) solution in combinations with the mineral N-fertilization. Two field experiments were carried out in a split–plot design with three replicates including a control treatment (without application). The main factor (F1) was the mineral nitrogen (N) fertilization in the ammonium nitrate form applied at the rates: 50%, 75%, and 100% of the recommended dose (RD). The sub-factor (F2) was the Chs soak and/or spray treatments. Aqueous Chs solution was prepared (20 mL Chs diluted in 10 L water was used for a 24 h soaking time of the grains. A similar solution was sprayed on the soil 50 and 70 days after cultivation. Wheat grains (Triticum aestivum L.) were sown during November 2021 and 2022. After harvesting and analysis of soil and plant samples, the results have indicated that soaked grains combined with the 75% rate has showed the most significant increase in the soil available N by 120.4%, P by 1128.9%, and K by 68.1% compared to the control. Spraying the Chs has maximally increased the yield (ton kg-1) of the wheat grains significantly by 59.4%, 71.8% and 84.7%, respectively, with the nitrate rates 50, 75, and 100% RD compared to the control. Spraying also showed the most significant increase in N, P, and K uptake (kg ha-1) by grains was by 245.1, 194.6 and 192.6%, respectively at 75% N rate. The N, P, and K use efficiency (NUE) showed maximum values by soaking the wheat grains in the Chs solution before cultivation with the 75% N rate. The AE values of the Chs spraying are greater than the Chs soaking being linked to the wheat yield that may be attributed to repeating the spraying after cultivation during the plant growth stages rather than one soaking stage