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Kiwifruit (Actinidia deliciosa) is an emerging fruit crop in Nepal. However, owing to its poor seed germination, growers lack the techniques for high-quality quality uniform rootstock production for grafting purposes. Ten treatments were tested, including control, tap water, cow urine (5% and 10%), vermiwash (10% and 20%), KNO, (1% and 3%), and GA, (4000 ppm and 6000 ppm), using a Completely Randomized Design with three replications. The results revealed that the highest germination percentage (47.69%) was recorded on GA, at 6000 ppm, which was statistically at par with KNO, 1%, GA, 4000 ppm, KNO, 3%, and vermiwash 20% and the lowest germination percentage (35.07%) was recorded on the control. The highest seedling height (9.74 cm) was recorded on KNO, at 3% which was statistically similar to KNO, 1%, vermiwash 20%, GA3 4000 ppm, and cow urine 10% and the shortest seedling height (6.69 cm) was found in the control. The highest fresh shoot weight (0.183 g), fresh root weight (0.093 g), dry shoot weight (0.019 g), dry root weight (0.005 g), and shoot length (3.86 cm) were observed in KNO, at 3% which was statistically at par with GA, 6000 ppm and vermiwash 20% and the lowest values recorded in the control. Similarly, KNO, 1% achieved the highest root length (6.06 cm) and seedling vigor index-1 (445.77), while the lowest values were recorded in the control. The highest seedling vigor index-11 (1.070) was found in GA, 6000 ppm, while the control showed the lowest (0.545), From the findings of this experiment, it is revealed that the seeds treated with GA3 6000 ppm, GA3 4000 ppm, KNO3 3%, KNO3 1%, vermiwash 20% and cow urine 10% could be the most effective and appropriate method to improve the germination and seedling growth of kiwifruit for rootstock purposes.